How do you make 1 BSA in PBS?
To make a 1% BSA solution in PBS-Tween, add 0.3 g BSA in 30 ml of PBS-Tween and put at 4 C for 10 minutes.
How do you get 2% BSA on PBS?
2% dry skimmed milk in PBS For one plate dissolve 0.2 g of powder in 10 mL of 1X diluted PBS. 5. 1% BSA in PBS For one plate dissolve 0.2 g of BSA in 20 mL of 1X diluted PBS. 6.
How do you make a 5% BSA solution?
For example, to make a 100mL 3% BSA solution, use 3 grams of BSA and bring the volume to 100mL. To make a 5% solution of BSA in 500mL of media (which is the typical media bottle size) 25grams of BSA would be used, brought to a volume of 500mL with the media of interest.
How do you sterilize BSA?
BSA (bovine serum albumin), 10% (w/v) Filter sterilize using a low-protein-binding 0.22-µm filter. Store indefinitely at 4°C. Lower-concentration stock solutions (e.g., 1%), which are useful for various applications, can be made by diluting 10% stock appropriately with sterile water.
How long can you keep BSA solution?
Lyophilized BSA although stable at room temperature for 3 weeks, should be stored 2-8°C. Upon reconstitution BSA should be stored at 4°C between 2-7 days and for future use below -18°C.
Can you filter sterilize BSA?
Should you filter BSA?
BSA can be used to detect phosphorylated proteins. However, BSA preparations contain tyrosine phosphorylations and will cause high background noise with anti-phosphotyrosine antibodies. Filter BSA before use. Unfiltered BSA may contain contaminating IgG or serum proteins which can cause background noise.
How do you make a 10% BSA solution?
For a 10% (100 mg/mL) stock solution of BSA, dissolve 1 g powdered Fraction V or molecular biology grade BSA in 10 mL of distilled H2O; to avoid clumping, dissolve by layering the powder on the surface of the liquid. Gently rock the capped tube until the BSA has dissolved completely.
Which is the best immunofluorescence treatment for BSA?
Quenching solution: 50 mM NH4Cl in PBS or 0.1M Glycine in PBS 4. Blocking solution 1% BSA or 10% FCS (fetal calf serum) in PBS 5.
How to dissolve 1% BSA in PBS tween?
To make a 1% BSA solution in PBS-Tween, add 0.3 g BSA in 30 ml of PBS-Tween and put at 4 C for 10 minutes. very easy! Nobody says this. For instance, you want to make a 5% solution in TBS-Tween. You just put 2.5 g BSA in 50 ml of TBS-Tween, you put at 4 C and after 10 minutes it is dissolved.
How to incubate a western blot in BSA?
For Western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Products available from Cell Signaling Technology are linked by their respective catalog numbers. NOTE: Prepare solutions with Milli-Q or equivalently purified water.