What are ion pairing reagents?
Ion Pairing Reagents are use as mobile phase additives, which allow the separation of ionic and highly polar substances on reversed phase HPLC columns.
What is ion pairing chemistry?
Ion pairing describes the (partial) association of oppositely charged ions in electrolyte solutions to form distinct chemical species called ion pairs.
What detectors are used in ion-pair chromatography?
UV detectors are widely used. Therefore ion-pair reagents must lack UV absorption themselves to obtain highly sensitive detection of samples. The UV absorption of sodium alkanesulfonates and quaternary ammonium salts is minimal so that these reagents can be used for reliable HPLC analysis.
Is formic acid an ion-pair?
The most commonly utilized ion-pair reagents are formic or acetic acids. Commonly concentrations are 0.05 to 0.2% for formic acid, and 0.3% for acetic acid.
What is ion pairing in HPLC?
In ion pair chromatography, the solute ion is distributed between the mobile and the stationary phase together with an ion of opposite charge (a so-called counterion). The technique is often used in reversed-phase chromatography as a convenient method to control the retention of solutes.
What are the disadvantages of ion exchange chromatography?
the exact mechanism of this technique is still elusive.
What is the principle of ion exchange chromatography?
Ion exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their affinity to ion exchangers. The principle of separation is thus by reversible exchange of ions between the target ions present in the sample solution to the ions present on ion exchangers.
What is an ion pairing agent?
Ion-pairing agents are compounds that contain both an ionic functional group and a hydrophobic portion, such as a hydrocarbon chain.
What is ion exclusion chromatography?
Ion Exclusion. Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later.
https://www.youtube.com/watch?v=BVvmjANaXBA
