What is pUC19 plasmid used for?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
What is the pUC19 plasmid?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. NEB offers a selection of common cloning plasmids and DNAs for use as substrates.
How was pUC19 constructed?
pUC19 is a small, high copy cloning vector for replication in E. coli. It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322. Inserts cloned into this site disrupt beta -galactosidase activity and give rise to white colonies on X-Gal/IPTG plates.
Who discovered pUC19?
Joachim Messing
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “plasmid”) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.
Where does the name pUC19 plasmid come from?
What is pUC19 plasmid? pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “ plasmid “) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.
Who is the creator of the pUC19 vector map?
From Wikipedia, the free encyclopedia Vector map of pUC19 pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
Where does the resistance gene pUC19 come from?
In addition to β-galactosidase, pUC19 also encodes for an ampicillin resistance gene (amp R), via a β-lactamase enzyme that functions by degrading ampicillin and reducing its toxicity to the host. The ori site, or origin of replication, is derived from the plasmid pMB1.
How many base pairs are in a pUC19 molecule?
The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1). NEB offers a selection of common cloning plasmids and DNAs for use as substrates.
