What is the composition of DMEM media?

What is the composition of DMEM media?

11965 – DMEM, high glucose

Components Molecular Weight Concentration (mg/L)
Amino Acids
Potassium Chloride (KCl) 75.0 400.0
Sodium Bicarbonate (NaHCO3) 84.0 3700.0
Sodium Chloride (NaCl) 58.0 6400.0

Is DMEM F12 serum free?

Use a base medium that supports serum-free growth. DMEM/F12 is a good base medium for most cell lines. DMEM, MEM and RPMI lack some of the micro-nutrients that are often required for serum-free growth. The F12 portion contains many of the micro-nutrients that cells need for serum-free growth.

What is advanced DMEM?

Advanced DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology.

What is the full form of DMEM?

Gibco Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

What does HyClone DMEM / F12 liquid media do?

HyClone DMEM/F12 liquid media is a nutrient 1:1 mixture that supports cell growth in research and biomanufacturing cell culture processes.

Which is the best DMEM / F12 serum free medium?

DMEM / F12 Serum-Free Medium DMEM/F12 is a serum-free medium formulation for general use. This formulation is a 1:1 blend of DMEM and Ham’s F12 media supplied complete and ready to use with L-glutamine, Hepes, BPE and EGF for culturing a wide range of cell types. DMEM/F12 does not contain phenol red or antibiotics.

Are there any antibiotics in Iscove DMEM F12?

DMEM/F12 does not contain phenol red or antibiotics. Iscove’s Modified Dulbecco’s Medium (IMDM) is a standard serum-free medium. It is supplemented with bovine pituitary extract and epidermal growth factor and contains L-glutamine and Hepes but no antibiotics.

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