How do you calibrate an eyepiece micrometer?
Line up the micrometer with your eyepiece reticle scale. Place the stage micrometer on the stage of the microscope and bring the scale into focus. Position the stage micrometer so that its zero overlaps with the zero on the eyepiece reticle scale, as I’ve shown here. Onto the third step of calibrating your objectives.
Why eyepiece micrometer is calibrated and how?
So it is in focus with image of the specimen. To calibrate the eyepiece graticule with a particular objective the stage micrometer is viewed through the microscope. When calibrating it is essential to ensure the same settings on the viewing head are used for calibration and subsequent measurements.
How do you calibrate an eyepiece reticle?
Linear measurements performed with a compound optical microscope generally cover a length range of approximately 25 millimeters down to 0.2 micrometer, upper and lower boundaries that are determined primarily by the maximum viewfield diameter and the microscope limit of resolution.
How is a stage micrometer used to calibrate an eyepiece?
This is done using a stage micrometer. These have an accurate scale on them on 1mm divided into 100 parts. So each small division on a stage micrometer = 0.01mm = 10µm To calibrate the eyepiece graticule with a particular objective the stage micrometer is viewed through the microscope.
What do you need to know about micrometer calibration?
Micrometer Calibration 1 Eyepiece Micrometer. Eyepiece micrometers enable the size of a specimen to be measured. 2 Stage / Slide Micrometer. The stage or slide micrometer is used to calibrate the eyepiece micrometer. 3 Procedure. The stage micrometer is placed on the platform/stage of the microscope.
How do you calibrate an objective lens in a microscope?
With that being said, note that each objective lens you will be using will need to be calibrated individually. To start with, load your eyepiece graticule into the eyepiece of your microscope and set the stage micrometer in place so that it is centered under the objective just like any other slide.
Where do you put the micrometer in the eyepiece reticle?
After the micrometer has been placed behind the reticle with the sliders in Coarse mode, click on the Fine radio button and carefully match one of the micrometer rules (preferably a numbered 100-micrometer interval line) with the far left-hand rule of the eyepiece reticle (numbered ” 0 ” on the default reticle).
