What is the molecular weight of Akt?
56 kDa
Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
What is p Akt?
Phosphorylated Akt (p-Akt) was implicated in inducing signals to interfere with the cell apoptosis, and promoting abilities of proliferation and motility through a crucial mechanism, the activation of mTOR (mammalian target of rapamycin) [4, 5, 6].
What is Akt Ser473?
The activity of the protein kinase Akt is frequently dysregulated in cancer and is an important factor in the growth and survival of tumour cells. Akt activation involves the phosphorylation of two residues: threonine 308 (Thr308) in the activation loop and serine 473 (Ser473) in the C-terminal hydrophobic motif.
What is Akt cell signaling?
Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10).
What activates mTORC2?
The mTORC2–ribosome association is induced by insulin-stimulated PI3K signaling and is independent of protein synthesis. Although ribosome-bound mTORC2 might exist at MAM, the underlying mechanism by which ribosome association activates mTORC2 is unknown.
What is AKT in cell signaling?
Akt is a major mediator of cell survival through direct inhibition of pro-apoptotic proteins like Bad or inhibition of pro-apoptotic signals generated by transcription factors like FoxO. Akt is critically involved in the regulation of metabolism through activation of AS160 and PFKFB2.
How is Akt regulated?
The PI3K-Akt pathway regulates PTEN levels by affecting its transcription and activity. When the pathway is activated by insulin, insulin receptor substrate 1 (IRS-1) transcription is down-regulated, in a negative feedback loop via mTORC1 and S6K1 activation.
What is Akt treatment?
Akt 4 Kit is a combination medicine used in the treatment of tuberculosis. It prevents the growth of the microorganisms that cause the infection.
What kind of antibody is used for Akt?
Phospho-GSK-3β (Ser9) Antibody #9336 was used to confirm downstream pathway inhibition (B). Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® Akt siRNA I (+), using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108.
How is phospho-AKT antibody used in cell signaling?
Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). Flow cytometric analysis of LNCaP cells, untreated (green) or LY294002-treated (blue), using Phospho-Akt (Ser473) Antibody compared to a nonspecific negative control antibody (red).
How does Akt play a role in apoptosis?
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3).
What is the phospho-AKT pathway sampler kit?
The Phospho-Akt Pathway Antibody Sampler Kit provides an economical means to evaluate the activation status of the Akt signaling pathway, including PTEN and phosphorylated Akt, GSK-3beta, c-Raf and PDK1. The kit includes enough primary and secondary antibodies to perform two Western blot experiments.
