Is RNA polymerase II DNA dependent?
Eukaryotic RNA polymerase II (pol II) is a 12-subunit DNA-dependent RNA polymerase that is responsible for transcribing nuclear genes encoding messenger RNAs and several small nuclear RNAs (1).
What is the function of RNA polymerase II?
RNA polymerase II catalyzes the synthesis of precursor mRNA. In eukaryotes, this RNA is generally longer than the final or “mature” mRNA, whose molecule is used as a template for protein synthesis. Promoter. The promoter comprises three sites.
What genes does RNA polymerase II transcribe?
RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. It is one of the three RNAP enzymes found in the nucleus of eukaryotic cells.
Does RNA polymerase II unwind DNA?
RNA polymerase II allows unwinding and rewinding of the DNA and thus maintains a constant length of the transcription bubble. J Biol Chem.
How are anti RNA polymerase III antibodies used in SSC?
The detection of anti-RNAP III antibodies is useful in the diagnosis of SSc and for the identification of patients at risk for developing progressive skin thickening and renal crisis. The prevalence of IgG RNAP III antibodies is from 3 – 58% in SSc patients.
What are anti RNA polymerase III ( Ara ) antibodies?
Anti-RNA polymerase III antibodies (ARA) are a specific marker for Systemic Sclerosis (SSc), associated to severe disease with major organ and diffuse cutaneous involvement.
When to use RNA polymerase III ( IgG ) serum?
RNA Polymerase III Ab, IgG, S Useful For Evaluating patients suspected of having systemic sclerosis, when used in conjunction with centromere and Scl70 antibodies
Where does the Assembly of RNA polymerase II take place?
It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins. Part of the assembly of the holoenzyme is referred to as the preinitiation complex, because its assembly takes place on the gene promoter before the initiation of transcription.
