What is isoelectric point of antibody?
One important characteristic of monoclonal antibodies (mAbs) is their isoelectric point (pI), which essentially is the pH at which the antibody has no net electrical charge, and its value depends on the charged amino acids the antibody contains.
What is pI of IgG?
IgG is the main low molecular weight immunoglobulin present in mammalian serum, and makes up 75% of serum immunoglobulins. Isoelectric point 6.1-8.5 (7.3 +/-1.2) if monoclonal (polyclonal antibodies do not have a set isoelectric point as they are a pool of various classes).
How do you find the pI of a protein?
The charge state of the protein at a given pH is the sum of the negative and positive charges on the charged residues and the C-terminal and N-terminal residues of the protein. To determine the pI value for the protein, the pH value at which the charge state of the protein is equal to zero must be found.
How is the isoelectric point of a protein calculated?
Prot pi [prɑːt paɪ] | Protein Tool. Prot pi | Protein Tool calculates isoelectric point and net charge of proteins, as well as the exact mass and the absorption coefficient using the amino acid sequence. Isoelectric point, mass and retention time of proteins are affected by covalent modifications.
How are isoelectric points determined for monoclonal antibodies?
Isoelectric points (pI) were determined for 23 therapeutic monoclonal antibodies by icIEF. Experimental pIs were very well correlated with calculated pIs. Relative charge variants were successfully measured by CEX in pH and salt-gradient modes.
How are the isoelectric points of a MAB determined?
In this work, isoelectric points (p I s) of 23 Food and Drug Administration (FDA) and European Medicines Agency (EMA) approved mAbs were determined by imaged capillary isoelectric focusing (icIEF), and ranged from 6.1 to 9.4.
Which is the best IPC isoelectric point calculator?
For big datasets use standalone version or split your input into 50k chunks. Reference: Kozlowski LP (2016) IPC – Isoelectric Point Calculator. Biology Direct 11:55.
