What are the HPLC parts?
A basic analytical high-performance liquid chromatography system comprises several components, including an eluent reservoir, a pump, an injection valve, an HPLC column, a detector, and a PC with a liquid chromatography data system installed.
What are the different parts of a chromatography system?
Modern LPLC systems consist of a column, injector, low-pressure pump, detector, and often a fraction collector. The mobile phase is pulled into the chromatography system by the action of the pump. From there, it travels to the injector where the sample to be tested is added.
What is instrumentation of HPLC?
HPLC instrumentation is typically made up of nine basic components: mobile phase/solvent reservoir, solvent delivery system, sample introduction device, column, post-column apparatus, detector, data collection and output system, post-detector eluent processing, and connective tubing and fittings.
What is structure of HPLC?
High-performance liquid chromatography (HPLC) column consists of a tube, pressure cap, seal ring, filter, joint, screw, and the like. The components in the column are separated into the detector, and the chromatographic signal is recorded by a recorder, an integrator or a chromatographic workstation.
How many types of pumps are there in HPLC?
3
There are 3 main types of HPLC pumps: 1. reciprocating pump – 90% usage. The solvent is drawn into a small chamber (when the solvent check valve is open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor-driven piston (Fig 28-6 or R5 Fig 6.9).
What is the basic principle of chromatography?
Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase.
Which pumps used in HPLC?
Most HPLC pumps are reciprocating pumps. The solvent is drawn into a small chamber (with the solvent check valve open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor driven piston.
