How do environmental factors impact the catalase enzyme?
As the temperature rise, the enzyme reaction rate will also increase until a certain temperature and above that temperature, the reaction rate will decrease. Catalase is enzymes that convert the harmful byproduct of metabolism, hydrogen peroxide, into oxygen and water and is found in all living cells.
What factors affect catalase activity?
The rate at which an enzyme works is influenced by several factors including the concentration of substrate (hydrogen peroxide in the case of catalase), temperature, pH, salt concentration and the presence of inhibitors or activators. Every enzyme has an optimal range for each of these factors.
How can time affect enzyme activity?
The longer an enzyme is incubated with its substrate, the greater the amount of product that will be formed. As a result, the rate of formation of product slows down as the incubation proceeds, and if the incubation time is too long, then the measured activity of the enzyme is falsely low. …
How does temperature affect catalase enzyme activity?
Effects of Temperature As the temperature increases toward the optimum point, hydrogen bonds loosen, making it easier for catalase to act on hydrogen peroxide molecules. If the temperature increases beyond the optimum point, the enzyme denatures, and its structure is disrupted.
How does hydrogen peroxide affect catalase?
Hydrogen peroxide is converted into two harmless substances, oxygen and water with the help of the enzyme catalase, which speeds up the reaction. The more substrate molecules present, the more collisions happen, and more enzyme activity until all the active sites are full causing the reaction to slow down.
What is the definition of enzyme concentration?
Substrate concentration is the number of substrate molecules found in a particular solution, while enzyme concentration is the number of enzymes. One enzyme can only act on one substrate molecule at a time, so an increase in enzymes means that more substrate molecules can be converted into the products of the reaction.
What is catalase activity?
Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals). It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS).
