What are functional assays?
In this regard, functional assays can be defined as systematic in vivo experiments that are designed to determine the involvement of each protein in a particular cellular pathway or biological process.
What is a flow cytometry assay?
Flow cytometry is a technology that rapidly analyzes single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution. Each particle is analyzed for visible light scatter and one or multiple fluorescence parameters.
What are in vitro functional assays?
From Latin meaning in glass, in vitro assays are designed with components of cells that have been isolated to monitor biochemical and functional reactions to determine mechanism of actions and impact of novel therapeutics.
What is a functional assay pharmacology?
Stated simply, a drug binds to a receptor and then “does something.” It is the purpose of functional assays to measure and quantitate that “something,” with the choice and design of assay being highly dependent upon the type of receptor (i.e., GPCR or ion channel), as well as the effect being studied (agonism.
What do the results of my flow cytometry mean?
What Do the Results Mean? Flow cytometry can provide information that is used to diagnose, stage and monitor blood cancers . It can also be used to test for minimal residual disease (MRD), the number of cancer cells remaining in the body after treatment.
What do you use flow cytometry for?
Flow cytometry is used to determine the physical and chemical properties of cells in a heterogeneous population.
What types of samples can be used for flow cytometry?
The most straightforward samples for flow cytometry include non-adherent cells from culture, waterborne microorganisms, bacteria, and yeast. Even whole blood is easy to use – red cells are usually removed by a simple lysis step.
What does flow cytometry measure?
Flow cytometry. Flow cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. A sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.
