What does Sirius red stain?
Sirius Red F 3B (Direct Red 80) is an azo dye primarily used in staining methods for collagen and amyloid. It has the molecular formula C45H26N10Na6O21S6. Collagen fibers in red. Muscular fibers in yellow.
How do you make Sirius red stain?
Methods. During the heating process, prepare picro-sirius red stain solution (PSR). The recipe is as following:0.1% direct red 80 plus 0.1% fast green FCF dissolved in saturated aqueous picric acid (1.2% picric acid in water), the operation needs about 20 min, so it could finished before deparaffinize steps.
How does Picrosirius red work?
Relatively inexpensive, the technique relies on the birefringent properties of collagen molecules. While the picrosirius red stain alone does not selectively bind collagen network, it becomes more specific than the other common collagen stains when combined with polarized light detection.
What stains collagen red?
Van Gieson’s stain in an angioleiomyoma, making smooth muscle fibers yellow and collagen fibers red. Hematoxylin and Van Gieson’s stain gives collagen a pink color, such as in fibrosis (arrows, here in cirrhosis).
What are the advantages of Sirius red staining?
Sirius Red staining is presented as a method for collagen determination, enabling quantitative morphometric measurements to be performed in locally defined tissue areas. The advantage of this method is especially shown for alveolar lung tissue. By excluding the bronchial areas in the tissue sections …
When to use Picro Sirius red stain kit?
Picro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is intended for use in the histological visualization of collagen I and III fibers in addition to muscle in tissue sections. The picro sirius red staining may be viewed using standard light microscopy. Picro sirius red staining has traditionally sometimes been used…
What causes de-staining of nuclei in picro-Sirius red?
The long time in picro-sirius red causes appreciable de-staining of the nuclei. This is not a problem with traditional van Gieson or with picro-aniline blue, with their 1-minute staining times.) When examined through crossed polars the larger collagen fibers are bright yellow or orange, and the thinner ones, including reticular fibers, are green.
What are the results of staining collagen nuclei?
Clear in xylene and mount in a resinous medium. Results: In bright-field microscopy collagen is red on a pale yellow background. (Nuclei, if stained, are ideally black but may often be grey or brown. The long time in picro-sirius red causes appreciable de-staining of the nuclei.
