What is the role of thymidylate synthase?
Thymidylate synthase plays a central role in the biosynthesis of thymidylate, an essential precursor for DNA biosynthesis. In addition to its role in catalysis and cellular metabolism, studies from our laboratory have shown that thymidylate synthase functions as an RNA binding protein.
What are the substrates and products in a reaction that is catalyzed by human thymidylate synthase?
Thymidylate synthase (TS) (EC 2.1. 1.45) is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). Thymidine is one of the nucleotides in DNA. With inhibition of TS, an imbalance of deoxynucleotides and increased levels of dUMP arise.
What reaction does the enzyme thymidylate synthase Catalyse?
The enzyme thymidylate synthase (TSase), an important chemotherapeutic drug target, catalyzes the formation of 2′-deoxythymidine-5′-monophosphate (dTMP), a precursor of one of the DNA building blocks.
What is Deoxythymidylate synthesis?
The synthesis of deoxythymidine from thymine in cell-free systems prepared from human leukocytes is mediated by two enzymatic mechanisms, pyrimidine deoxyribosyI transferase and deoxythymidine phosphorylase. Both activities are probably a function of one protein.
What happens when thymidylate synthase is inhibited?
Thymidine is one of the nucleotides in DNA. With inhibition of TS, an imbalance of deoxynucleotides and increased levels of dUMP arise. Both cause DNA damage.
How big is the catalytic domain of thymidylate synthase?
Thymidylate synthase is an enzyme of about 30 to 35 kDa in most species except in protozoan and plants where it exists as a bifunctional enzyme that includes a dihydrofolate reductase domain. A cysteine residue is involved in the catalytic mechanism (it covalently binds the 5,6-dihydro-dUMP intermediate).
What kind of enzyme is thymidylate synthase EC 2.1.45?
From Wikipedia, the free encyclopedia Thymidylate synthase (TS) (EC 2.1.1.45) is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). Thymidine is one of the nucleotides in DNA. With inhibition of TS, an imbalance of deoxynucleotides and increased levels of dUMP arise.
Which is a product of thymidylate synthase ( dTMP )?
Both cause DNA damage. The following reaction is catalyzed by thymidylate synthase: By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product.
